Document Details

Document Type : Thesis 
Document Title :
EFFECT OF DICLOFENAC ON DOXORUBICIN-INDUCED APOPTOSIS IN BREAST CANCER CELLS
تأثير عقارالدكلوفيناك على موت الخلايا ا المبرمج الذي يحدثه الدوكسوروبيسين في خلايا سرطان الثدي
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Background: Breast cancer is the most common disease in women worldwide. Doxorubicin (DOX) is a chemotherapeutic drug from anthracycline family and is highly effective in advanced breast cancer. Unfortunately, DOX may lead to severe heart damage. Many strategies have been investigated to protect the heart against DOX induced cardiotoxicity. Recent studies evaluated the effect of diclofenac (DICLO)as an anti-cancer. Therefore, the current study aimed to investigate the potential chemo sensitizing effect of Diclofenac on DOX cytotoxicity on MCF-7 as well as elucidating the possible underlying mechanism. Materials and methods: Evaluate the effect of DOX on apoptosis induction, cell cycle phase distribution, cellular absorption, and p-glycoprotein activity in breast cancer cells when DICLO was present with different concentrations. Results: The addition of DICLO (10 and 100 µg / ml) increased the effectiveness of DOX as it led to a significant decrease in IC50 compared to the cells treated with DOX alone. Cell cycle analysis showed that DICLO (100µg / ml) significantly increased the percentage of apoptotic phase cells in DOX (0.25 µg / ml) from 3.5% to 10.9%.In addition, significantly increased apoptotic phase cells to 40.2%when DICLO added to DOX(1 µg / ml) compared to 15.6% in DOX alone. Programmed cell death test showed that combined treatment of DICLO with DOX resulted in a significant increase in the rate of early programmed cell death to more than 12 times and three times greater than DOX(0.25 µg / ml and 1µg / ml) respectively. DICLO increased the cellular absorption of DOX significantly by 32% and 63% compared to DOX alone (5 µg/ ml and 10 ug/ml) respectively. The addition of 100 μg/mL diclofenac 10 μg/mL DOX re Conclusion: The present study concluded that DICLO potentiates the cytotoxic activity of DOX against the growth of MCF-7 cancer cells through induction of apoptosis, inhibition of p-glycoprotein activity and enhance DOX cellular uptake. 
Supervisor : Prof. Abdel-Moneim M. Osman 
Thesis Type : Master Thesis 
Publishing Year : 1442 AH
2021 AD 
Co-Supervisor : Dr. FatemahOmar Kamel 
Added Date : Monday, June 7, 2021 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نهي الديب محمدMohammed, Noha Al-DeebResearcherMaster 

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