Document Details
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Document Type |
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Thesis |
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Document Title |
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EFFECT OF THYMUS VULGARIS OIL AGAINST THE HELICOBACTER PYLORI-INDUCED GASTRIC ULCER IN RATS تأثير زيت الزعتر البري علي قرحة المعدة المستحدثة تجريبا بالبكتيريا الحلزونية في الجرذان |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Stomach ulcer is a common disorder of the gastrointestinal tract, which affects many people worldwide, with many side effects and sufferings to these patients. About 50% of these ulcers are caused by Helicobacter pylori (H. pylori) infection, which is also the main etiology of gastric ulcer, chronic gastritis, and gastric tumors. The treatment of H. pylori is typically based on a combination of at least two antibiotics in combination with gastric acid inhibitors. Triple Therapy and Bismuth Quadruple Therapy are well-known therapeutic measures used in the eradication of H. pylori and H. pylori-induced gastrointestinal disorders. The current work was designed to evaluate the gastroprotective effect and the antioxidant of Thymus vulgaris essential oil (TVEO) against H. pylori-induced gastric ulcer in rats and to compare these effects with another model of indomethacin-induced gastric ulcer. Also, the role of this oil was compared with another common drug Ranitidine that is used in the treatment of gastric ulcers. Seventy-two male adult Sprague-Dawley albino rats weighing 190-220 rats were used in this study, which was randomly divided into nine groups (8 rats each). The rats of the 1st three groups received distilled water, TVEO, and ranitidine respectively and regarded as control groups (G 1, 2 and 3); in the rats of the 4th and 5th groups, the gastric ulcer was induced using H. pylori model (G 4) and indomethacin model (G 5); while in the rats of the 6th and 7th groups, the gastric ulcer was induced using H. pylori model and was given either TVEO (G 6) or ranitidine (G 7) as a protecting agent respectively. In the last two groups, the gastric ulcer was induced using the indomethacin model, which was given either TVEO (G 8) or ranitidine (G 9) as a protecting agent respectively. At the end of the experimental period of different groups, the rats were sacrificed where the abdomen was opened, and the stomach was dissected out. Then, the stomach was opened, and the gastric content was collected for the estimation of the acid secretory parameters such as the volume of gastric juice, pH, free and total acidity, mucus production, and pepsin concentration. In addition, gross changes were assessed, and ulcer index was calculated. Also, the oxidative stress and antioxidant parameters were investigated in the gastric tissue using the following indicators: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Moreover, the histopathological examination of gastric mucosa using different stains and immunohistochemical localization for Ki-67 was performed. Finally, all data were recorded and statistically analyzed. The results of this study showed that in both H. pylori and indomethacin-induced ulcer models, there were alterations in all measured gastric secretory parameters such as the volume of gastric juice, free and total acidity, pepsin concentration and mucin content. Moreover, there was a marked elevation in oxidative stress marker (MDA) with a significant decrease in the antioxidant enzymes DOS, CAT, and GPx. The macroscopic and histological appearance of the gastric mucosa displayed variable degrees of gastric damage with decreased expression of Ki-67 immuno-reaction in both ulcer models. The histopathological observations also confirmed the healing effect of TVEO against the gastric mucosal damage in the two models, which was nearly or even better than that ranitidine treated group. This study showed the antiulcerogenic and gastroprotective effects of TVEO in H. pylori and indomethacin-induced gastric ulcers in rats. The protective effect of TVEO could be attributed to the gastric acidity and increased mucus production and antioxidant activities. However, further studies are required to isolate the active compounds from TVEO and to elucidate the exact mechanism of their action on the stomach.
Keywords: Protective effect; Thymus; vulgaris; essential oil; against H. pylori induced; gastric ulcer; rats |
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Supervisor |
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Prof. Mohamed Morsi M. Ahmed |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1442 AH
2021 AD |
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Co-Supervisor |
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Dr. Gamal Said Abd El-Aziz |
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Added Date |
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Wednesday, June 23, 2021 |
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Researchers
| عبد العزيز عبدالله المانع | Almanea, Abdulaziz Abdullah | Researcher | Doctorate | |
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