Document Details
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Document Type |
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Thesis |
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Document Title |
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Investigating the Biochemical and Anticancer Properties of Natural Product Allicin in Red Blood Cells and Leukemic Cells دراسة الأثر الكيميائي الحيوي والمضاد للسرطان لمادة الاليسين على خلايا الدم الحمراء وخلايا سرطان الدم |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Allicin (ACN), a sulfoxide in freshly crushed garlic, it is formed by an enzymatic reaction by the action of alinase. Alinase and alliin take place in many parts of the garlic compartments and hence uncut garlic have no ACN but it contains alliin -its precursor- instead, also it is known for its diverse bioactive properties. Among the most notable effects of ACN is its antitumor activity demonstrated against a wide array of cancer types. Thus, ACN may be a promising anticancer therapeutic. Nevertheless, chemotherapy-induced anemia is a major obstacle in cancer management with a prevalence of up to 70%. Although the pathophysiology behind it remains elusive, a number of medications known to cause anemia in patients have been demonstrated to induce premature apoptosis in RBCs known as eryptosis. The present study, thus, explore the toxic effects of ACN on human RBCs and delineate the underlying biochemical mechanisms.
Hemoglobin leakage was used as a surrogate for hemolysis which was photometrically measured. Major eryptotic events were examined using flow cytometry with fluorescent probes. Exposure to phosphatidylserine (PS) was tested by Annexin-V-FITC, cytosolic calcium with Fluo4/AM, and reactive oxygen species (ROS) with H2DCFDA.
Our results show that ACN induces hemolysis in a dose-dependent fashion, which is significantly abrogated in absence of extracellular calcium. Moreover, ACN stimulates phosphatidylserine exposure, intracellular calcium overload, and oxidative stress. Using small-molecule inhibitors, we demonstrate that the pro-eryptotic activity of ACN is ameliorated in presence of zVAD(OMe)-FMK, SB203580, and D4476.
ACN possesses hemolytic and eryptotic properties mediated through elevated intracellular calcium levels, oxidative stress, caspase, p38 MAPK, and CK1α.
Keywords: Allicin (ACN), programmed cell death, Phosphatidylserine (PS), Calcium, CK1α |
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Supervisor |
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Dr. Samar Sultan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2021 AD |
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Added Date |
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Friday, August 13, 2021 |
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Researchers
| محمد حسن خواجي | Khawaji, Mohammed Hassan | Researcher | Master | |
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