Document Details

Document Type : Thesis 
Document Title :
INFLUENCE OF AGE ON ISOLATION AND CULTURE OF MOUSE BONE MARROW MESENCHYMAL STEM CELLS
تأثير العمر على فصل و زراعة الخلايا الجذعية الميزينشيمية من نخاع العظم للفأر
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Bone marrow mesenchymal stem cells (BMMSCs) can be the origin of numerous tissues such as fat, bone, and cartilage. Further, they can be mobilized and intervene in damaged tissue contributing to their regeneration. However, no evidence has proved that BMMSCs may initiate any primary tumor or other serious harmful effects. In this study, we aim to evaluate the effect of age on BMMSCs in mice. The BMMSCs were isolated from mice at different ages (3, 6, and 9) weeks old and cultured in DMEM medium and characterize them cells using MSC-related Markers (CD29, CD34, CD44, CD45, CD73, CD105) via flow cytometry. Besides, the proliferation condition of cells was assessed by MTT assay in the early passage (P3) at different days of culture including 3, 9, and 15 days. Our results showed that an increase of BMMSCs numbers in the three age groups and having different shapes of the cells during the culture days at the initial passage on the 3rd day until the 20 days, on the selected age. In addition to strong expression of MSC markers. Furthermore, the number of BMMSCs derived from mice at 3 weeks old had a higher and proliferation rate than did those derived from older mice. While the number of cells was similar in 6th weeks and 9th weeks old mice at the 15th-day duration of culture. In conclusion, the BMMSCs maintained the MSC characteristics in all study groups and increased their numbers in the early stage of life compared to the later stages of life this might due to the aging of mice. The direct relationship between age and numbers of cells in the mice still needs further pieces of evidence. However, it is still important to identify the genotypic characteristics of MSCs via gene expression patterns in the different stages of mice life. 
Supervisor : Prof. Dr. Saleh Abdul Aziz Alkarim 
Thesis Type : Master Thesis 
Publishing Year : 1443 AH
2022 AD 
Added Date : Sunday, March 6, 2022 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد يسلم الدخينيAldkhaini, Mohammed YaslamResearcherMaster 

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