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Document Type |
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Thesis |
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Document Title |
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Proteomic analysis and antimicrobial potentials of Milk Fat Globular Membrane (MFGM) Proteins of Saudi (Safra and Wadha) Camelus dromedary clans تحليل المحتوى البروتيني والقدرات المضادة للميكروبات لعضيات الحليب الدهنية لعشائر (الوضحاء والصفر) الإبل السعودية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Milk fat globule membrane (MFGM) proteins have several benefits for infants. Our research studied and analysed the MFGM proteins for two of the Saudi camel clans Camelus dromedarius (C. dromedarius) (Safra and Wadha) throughout the lactation cycle, as well as the heterogeneity of MFGM proteins at the interclan and intraclan levels. The study also examined the ability of MFGM to inhibit two types of bacterial microbe strains Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The sampling phase was carried out in four sets during the four seasons of the year at different locations in Jeddah city, where 105 samples were collected in total. MFGM protein ingredients were extracted and estimated by protein analysis method “Bradford protein analysis”. The morphology and sizes of MFGM were also studied using the Scanning Electron Microscope (SEM). The protein profiles of MFGM and the differences in the interclan and intraclan levels were studied and investigated using SDS-PAGE, SDS-UREA-PAGE and Proteomic analysis. The presence of some key proteins in camel MFGM, was also determined using ELISA analysis. Finally, the MFGM antimicrobial activity was tested using diffusion test. Our results showed that MFGM size of Arabian camel ranged from 1 to greater than 5 µm in diameter. In vitro, the presence of some main camel MFGM proteins was documented such as: butyrophillin (Btn), Lactadherin (LDH), Vinculin, Lactoferrin (LF), Acetylserotonin O-methyltransferase, Septin-6, Casein kinase CK, Actin, Mucin (MUC), Fatty acid-binding protein (FABP) and CK1 that are present in different concentrations depends on different camels, clans and seasons. Moreover, results of MFGM proteins analysis showed that there were variance protein concentrations for Safra camel clan compared to Wadha. Our results clearly revealed that there are multiple differences in the interclans and intraclans levels in the protein profiles as observed in SDS-PAGE. Proteomic analysis also confirmed this outcome. Although, the antimicrobial results do not reveal any effect of MFGM in comparison with antibiotic and camel LF protein results. This study has extended our knowledge that there are major, minor and redundant proteins spots. |
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Supervisor |
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Prof. Elrashdy M. Redwan |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1443 AH
2022 AD |
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Added Date |
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Monday, January 16, 2023 |
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Researchers
| بسام حسن سبحي | Sabha, Bassam Hassan | Researcher | Master | |
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