Document Details

Document Type : Thesis 
Document Title :
Assessing The CpG Methylation and Transcription Factors Binding Sites in The Promotor Region of Ras Like Oncogene B (RALB) in The Saudi Autistic Children
تقييم مستوى مثيلة الحمض النووي ومواقع ارتباط عوامل النسخ لمنطقة البروموتر في جين RALB في أطفال التوحد السعوديين
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Aberrant DNA methylation has been observed in patients with autism spectrum disorder (ASD). Autism is a group of complex neurodevelopment disorders described as impairment in social interaction and reciprocity, and repetitive in speech. DNA methylation (DNAm) is one of the common epigenetic modifications, which take place in biological processes. It has a crucial role in regulating gene expression through controlling transcription factor binding sites (TFBSs) within gene promoter. Recent studies have reported many genes exhibit differences in gene expression levels among autistic subjects. Most of these genes are implicated in vital biological process such as signal transduction and neurodevelopmental mechanisms. RAS Like Proto-Oncogene B (RALB) gene belongs to GTPase superfamily, which is involved in various cellular processes including exocytosis, neuron branching and migration. Therefore, we hypothesized that ASD could be associated with aberrant DNAm in the TFBS of RALB promoter. In this study, the MethyLight was used for examining the CpG site within RALB promoter. RT-qPCR was measured the RALB expression in peripheral blood mononuclear cells from siblings of 19 autistic children and 18 healthy children. The children's age is ranging from three to Twelve years old. Moreover, the Genomatix MatInspector software was used to determinate the standard transcription factor (TF) in RALB function. Although the DNAm undetected in RALB promoter, we found an increased RALB expression in 11 autistic subjects relative to the controls. The E2F3 was selected as a potential TF, interestingly seven of up regulated samples were consistent with up regulation of E2F3 expression. The differentiation expression of RALB in autistic children represents potential evidence of the aberrant DNAm in other CpG sites has ability in regulating the E2F3 binding affinity, and this could be related to the development of ASD. 
Supervisor : Dr. Khloud Algothmi 
Thesis Type : Master Thesis 
Publishing Year : 1443 AH
2022 AD 
Added Date : Saturday, January 28, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مريم سالم العتيبيAlotaibi, Maryam SalemResearcherMaster 

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