Document Details
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Document Type |
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Thesis |
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Document Title |
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Extraction of thymoquinone-rich volatile oil of Saudi Arabian black cumin seeds and screening of its anticancer activities استخلاص زيت طيار غني بثايموكينون من بذور الحبة السوداء ( حبة البركة ) المحلية وفحص فعالياته المضادة للسرطان |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Black seed oil (BSO), also known as Nigella sativa oil, is a well-known Mediterranean cuisine that has been linked to improved human health. Thymoquinone (TQ), the pharmacologically active component of BSO, is largely responsible for its therapeutic effects. TQ targets several epigenetic players, including the ubiquitin-like-containing plant homeodomain (PHD), an intriguing new gene (RING finger domains 1), DNA methyltransferase 1 (DNMT1), and HDAC1 to inhibit cell proliferation and induce apoptosis (histone deacetylase1). This project's goal was to extract BSO. From black seeds obtained from local Al- Qassim's market in Saudi Arabia, and to ascertain its TQ content. After that, the isolated BSO's inhibitory effects on the expression of UHRF1, DNMT1, and HDAC1 in various cancer cells and the associated events were assessed. The same tests were also run on a pure TQ for comparison. A supercritical fluid extraction device was used to extract BSO. The components of the extracted BSO were profiled, and the extraction effectiveness of the bioactive chemicals in the extracted BSO was assessed, using a GC-MS system. TQ detection and quantification were done using HPLC. The WST-1 Cell Proliferation test and the Annexin V Binding Guava Nexin were used to measure the effects of the isolated BSO during a 24-hour period on cell proliferation and apoptosis, respectively. The effect of the extracted BSO on the expression of the trimeric complex UHRF1/DNMT1.HDAC was investigated using RT-qPCR. To assess the interactions of TQ with UHRF1, DNMT1, and HDAC1, molecular docking and molecular dynamic (MD) simulation analyses were accomplished. Thymoquinone (5.9%) was the main volatile component in the extracted BSO, according to HPLC analysis. MCF-7, Hela, and Jurkat cell growth was markedly and dose-dependently reduced by BSO. BSO dramatically reduced the mRNA expression of UHRF1, DNMT1, and HDAC1 in cancer cells, causing apoptosis to occur as a result. TQ binds to UHRF1, DNMT1, and HDAC1 according to molecular docking and MD modelling. Our findings suggest that the direct targeting of UHRF1 and its companions DNMT1 and HDAC1 by TQ-rich BSO provides a promising approach for epigenetic therapy for both solid and blood cancers. |
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Supervisor |
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Dr. Mahmoud Alhosin |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2022 AD |
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Added Date |
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Monday, February 27, 2023 |
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Researchers
| صفي الدين رضا السنوسي | Alsanosi, Safialdeen Reda | Researcher | Master | |
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