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Document Type |
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Thesis |
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Document Title |
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Evaluation of Dry Fasting Effect on Protein Glycation in Saudi Individuals. تقييم تأثير الصيام الجاف على عملية ارتباط البروتين بالسكريات لدى الأفراد السعوديين |
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Subject |
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Faculty of medicine |
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Document Language |
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Arabic |
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Abstract |
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Introduction: The non-enzymatic protein glycation occurs between amine groups of protein and reducing sugar, which alters protein function and contributes to many biochemical disorders. The accumulation of advanced glycation end-products (AGEs) is associated with the complications of diabetes, kidney disease, metabolic disorders and degenerative diseases.
Objectives: This study was designed to measure the level of some glycated proteins such as glycated insulin and glycated hemoglobin in Saudi volunteers before and after dry fasting.
Methods: Blood samples were collected in two stages; first sample (before dry fasting) and second sample (after dry fasting) from 45 Saudi male volunteers, whose ages ranged between23 and 52 years with a mean value of 33.92 ± 8.1years. They were categorized into four groups: healthy, type II diabetic patients, smokers, and obese individuals. Plasma glucose level and HbA1c was measured immediately using (Beckman coulter). Assessment of insulin resistance was determined by using the equation of homeostasis model for insulin resistance (HOMA-IR). Plasma insulin levels and glycated insulin % was assayed by TSQ Vantage Triple Stage Quadruple LC/MS Mass Spectrometer from Thermo Scientific.
Results: Level of HbA1c, HOMA IR and glycated insulin in healthy, type II diabetic patients, smokers, and obese individuals showed a significant decrease in second sample (after dry fasting) compared to first sample (before dry fasting). The level of HbA1c showed a significant decrease (p-value <0.0001) after dry fasting (5.69 ± 1.55 %) compared to before dry fasting (6.14 ± 1.77%). The level of HOMA IR showed significant decrease (p-value <0.0001) after dry fasting (1.97 ± 1) compared to before dry fasting (2.46 ± 1.28). The level of glycated insulin showed significant decrease (p-value <0.0001) after dry fasting (6.25 ± 2%) compared to before dry fasting (7.71 ± 2.48%)
Conclusion: Dry fasting have been shown to improve various metabolic disorders in type II diabetic patients, smokers, and obese individuals such as decrease glycated insulin, HbA1c and HOMA. Dry fasting decrease accumulation of AGEs by reducing attachment of sugar or sugar derivative to a protein that involves in non-enzymatic glycation via enhance proteolysis and decreases protein synthesis. |
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Supervisor |
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Dr. Khalid Hussein Bakheit |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2022 AD |
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Added Date |
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Tuesday, March 7, 2023 |
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Researchers
| عبدالعزيز سعد العمري | ALAMRI, ABDULAZIZ SAAD | Researcher | Master | |
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