Document Details
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Document Type |
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Thesis |
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Document Title |
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Identification of a Bacterial Consortium from Red Sea Water Capable of Degrading Some Aromatic Hydrocarbon Compounds تعريف مجموعة بكتيرية من مياه البحر الأحمر قادرة على تحليل بعض المركبات الهيدروكربونية العطرية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Polycyclic aromatic hydrocarbons (PAHs) are considered one of the most important environmental problems at present, due to the presence of their compounds in many different environments and the significant pollution they cause in these environments. The bioremediation of polycyclic aromatic hydrocarbons is one of the most environmentally friendly, effective, and least expensive methods of removing pollutants from the environment. In this study, the bacterial consortium was enriched from water contaminated with petroleum hydrocarbon residues from the coast of Jeddah on the Red Sea - Saudi Arabia. The samples were enriched in a mineral salt medium, and benzene and anthracene were chosen as the sole carbon source. Some compounds were selected for the biodegradation experiments such as benzene, anthracene, naphthalene, and toluene at different concentrations between (50-1000 mg/L). The degradation rate of benzene (92.6%), anthracene (96.1%), naphthalene (97.6%) and toluene (95.2%) were recorded. A continuous stirred tank reactor (CSTR) was used in the lab to simulate the conditions required for bacterial growth in order to reveal the potential for treatment by bacterial consortium. The results of the chemical oxygen demand (COD) analysis revealed that 92.7% of the organic matter in the reactor was removed and the aromatic hydrocarbon compounds were degraded within 36 days. Bacteria were identified by molecular techniques using 16S rRNA, where the results showed that phylum Proteobacteria (Stenotrophomonas, Denitrobacter, Achromobacter, Acinetobacter, Haererehalobacter, Pseudomonas, Alcanivorax, Ochrobactrum, Parvibaculum, Marivita, Sphingobium, Martelella, and Thalassospira) dominated in benzene and anthracene used as the sole carbon source with percentages (56% and 76%), respectively, followed by phylum Actinobacteria (Bifidobacterium, Cellulomonas, Brevibacterium, Gordonia, and Corynebacterium) with a percentage of about benzene (42.75%) and anthracene (22%). The phyla Bacteroidetes and Firmicutes occupied a small proportion compared to the two previously mentioned. The results of this study revealed the ability of bacterial consortium to degrade PAHs and efficiently treat hydrocarbon-contaminated water under saline conditions. Therefore, these bacterial aggregates are a promising solution for treating and removing hydrocarbon compounds from environments contaminated with these compounds. |
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Supervisor |
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Dr. Bothaina Abdullah Ali Alaidaroos |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2023 AD |
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Co-Supervisor |
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Dr. Samyah Darwish Saddig Jastaniah |
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Added Date |
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Monday, March 20, 2023 |
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Researchers
| نوف حمد العيسى | AL-Essa, Nouf Hamad | Researcher | Master | |
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