Document Details

Document Type : Thesis 
Document Title :
PROFILING OF GUT MICROBIOME CONTAINING CARBAPENEM-RESISTANT ENTEROBACTERIACEAE IN INTENSIVE CARE UNIT PATIENTS
توصيف ميكروبيوم الأمعاء المحتوي على عائلة البكتيريا المعوية المقاومةللكاربابينيم في مرضى العناية المركزة
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : This study focused on the bacterial analysis in the stool samples of hospitalized patients suffering from carbapenem-resistant Enterobacteriaceae (CRE) infection. The overall hypothesis was to achieve a different complexity of species and certain biomarkers of gut colonization using culture-independent molecular biological approaches in comparison to conventional culture-based microbiology methods. Initially, 20 stool samples were obtained from the patients in the intensive care unit of King Abdulaziz University Hospital, Jeddah. DNA was extracted from stool samples that served as a PCR template to amplify phylogenetically informative 16S rRNA gene across the Domain Bacteria. PCR products were detected in all the studied cases. 20 CRE-positive and negative PCR products were sequenced by using Illumina MiSeq. The results demonstrated a similar bacterial richness between the two groups under antibiotic regimen. The operational taxonomic units (OTUs) reads profiling showed an average of four phyla (Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria) in both groups. The presence of Enterobacteriaceae and Pseudomonadaceae members was higher in the CRE-positive group whereas Bifidobacteriaceae and Sphingomonadaceae were commonly detected in the CRE-negative group. Keywords: CRE, gut microbiota, 16s rRNA gene, ICU patients, Colonization resistance. 
Supervisor : Dr. Nisreen Tashkandy 
Thesis Type : Master Thesis 
Publishing Year : 1444 AH
2023 AD 
Added Date : Tuesday, March 28, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سارة السيدAlsayed, Sarah ResearcherMaster 

Files

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 49128.pdf pdf 

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