Document Details
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Document Type |
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Thesis |
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Document Title |
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THE ANALYSIS OF BIOACTIVE PHYTOCHEMICAL OF PHRAGMANTHERA AUSTROARABICA AND THEIR POTENTIAL ANTI-BREAST CANCER EFFECTS تحليل المركبات النشطة كيميائيًا لنبتة الهدال وأثرها المحتمل كمضاد لسرطان الثدي |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Cancer is one of the leading causes of death and remains largely responsible for the worldwide decline in life expectancy. Therefore, chemoprevention in combination with phytochemicals is an emerging approach in cancer therapy. The aim of this study was to investigate Phragmanthera austroarabica as a source of cancer protective lead compounds. Phragmanthera austroarabica (Loranthaceae), a hemiparasitic herb, is widely known for its high content of polyphenolic compounds responsible for its antioxidant and anti-inflammatory properties. A colorimetric cytotoxicity assay was used to investigate the antiproliferative effects of two potential leaf extracts, ethanol and n-hexane. In comparison, the ethanol extract showed the highest effect with IC50 values of 60.71 and 23.02 µg/ml for 48 and 72 hours of treatment, respectively. The n-hexane extract showed a moderate effect (IC50 = 55.61 µg/ml) at the 72-hour treatment. Later, the extracts were subjected to GC-MS analysis, which revealed 10 potential phytochemicals. Moreover, in-silico molecular docking was applied to screen the most suitable candidate with an anti-apoptotic protein, the target protein. The analysis identified the five most active phytoconstituents by docking score (kcal/mol), namely beta-amyrin (-8.3), lupeol (-7.9), gamma-sitosterol (-7.6), vitamin E (-7.2), and benzophenone (-7.1). Computer-aided pharmacokinetic and toxicological studies were performed to demonstrate the bioavailability and safety profile of the phytochemicals. All compounds met the targeted parameters and proved to be potent candidates for cancer therapy. Therefore, P. austroarabica justified the anticancer effect on the breast cancer cell line via targeted apoptotic pathways. However, this study recommends further extensive laboratory studies to explore molecular mechanisms in the future.
Keywords: Phragmanthera austroarabica, Cytotoxicity, GC-MS, Molecular docking, ADME, Toxicity, Bcl-2 protein. |
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Supervisor |
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Dr. Salman Hawsawi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2023 AD |
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Added Date |
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Saturday, April 22, 2023 |
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Researchers
| مد عبد الأول | Abdel Awal, MD | Researcher | Master | |
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