Document Details

Document Type : Thesis 
Document Title :
Effect of DNA Methylation on RASA3 Protein Expression in Saudi Autistic Children
تأثير مثيلة الحامض النووي على التعبير البروتيني لـ (RASA3) لدى اطفال التوحد السعوديين
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Autism spectrum disorder (ASD) is a complex early-onset childhood neurodevelopmental disorder. ASD is marked by language deficit, social communication retardation, and stereotypical repetitive behaviors. The prevalence of ASD is increasing worldwide with approximately 1-2% cases from the general population with a higher male-to-female ratio of 4:1. The prevalence of ASD among the Saudi population is estimated 0.26%. ASD resulted from the combination of genetic and environmental factors that are scientifically called epigenetics. The epigenetic factors have four distinct mechanisms including: histone modification, chromatin remodeling, non-coding RNA, and DNA methylation. The exact role of these mechanisms of epigenetics on autism is still unclearly understood. Autism is considered one of the very challenging areas of study due to its complexity and comorbidity with other disorders. However, the search attempts of the Autism Research Team – Faculty of Science – Biology Sciences Department of King Abdul Aziz University reveal a group of genes has different methylation patterns in autistic children that differ from normal controls. One of the genes with aberrant methylation is RASA3. The recent research studied the effect of DNA methylation of RASA3 protein via western immunoblotting technique to demonstrate the RASA3 expression of six Saudi Autistic children and three normal controls. Unfortunately, the result was statistically non-significant (p-value = 0.16 ˃ 0.05). On the other hand, the previous result was considered biologically significant due to the smallness of the significance value. Recently, hypomethylation is the best explanation for the decreased expression of RASA3 in Saudi autistic children. In conclusion, the current study suggests the possibility of using oRASA3 protein as an earlier biological indicator for ASD. 
Supervisor : Dr. Mona Ghonaim Alharbi 
Thesis Type : Master Thesis 
Publishing Year : 1444 AH
2023 AD 
Co-Supervisor : Dr. Magdah Ali Ganash 
Added Date : Thursday, June 29, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
روان موسى سيدSayed, Rawan MousaResearcherMaster 

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