Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
Possible Effect of Vitamin D3 on Aortic Endothelial Nitric Oxide Synthase on Experimental Model of Metabolic Syndrome in Rat التأثير المحتمل لفيتامين د3 على إنزيم أكسيد النيتريك البطاني في الأورطى على النموذج التجريبي لمتلازمة الأيض في الجرذان |
|
Subject |
: |
Faculty of Science |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Metabolic syndrome (MetS) is characterized by visceral obesity, hypertension, insulin resistance (IR), type two diabetes mellitus (T2DM) and dyslipidemia which all increase the risk of cardiovascular disease (CVD). Nitric oxide (NO) is generated by endothelial nitric oxide synthase (NOS3) in vascular endothelial cells and it is essential molecule for cardiovascular function by acting as a vasodilator and systemic blood pressure (BP) regulator. The current study aimed to explore the alterations in aortic NOS3 expression in experimental model of MetS, and to further investigate the possible protective effects of vitamin D (Vit-D) on aortic NOS3 expression in this model. Thirty-six male Albino rats were placed randomly into 4 groups; control, Vit-D3 treated, MetS and MetS supplemented with Vit-D3. The experiment lasted for 12 weeks, body mass index (BMI) was recorded weekly, while BP was recorded every two week. At the end of the experiment, serum was used to assess lipid profile, insulin, glucose, tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6) and moreover IR was calculated. Furthermore, mRNA extraction was used to detect the relative expression of aortic NOS3. The results revealed that MetS significantly increased systolic BP, diastolic BP, glucose, cholesterol, triglycerides (TGs), low density lipoprotein (LDL), TNFα and IL-6. On the other hand, it showed a significant reduction in insulin, HDL and aortic NOS3 expression while no significant change was indicated in IR and BMI. Based on our results, Vit-D3 treatment elevated aortic NOS3 expression and mitigated MetS alterations adding to the current evidence concerning protective role of Vit-D against MetS complications. |
|
Supervisor |
: |
Prof. Ahlam Alahmadi |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1445 AH
2023 AD |
|
Co-Supervisor |
: |
Prof. Zienab Alrefaie |
|
Added Date |
: |
Saturday, September 30, 2023 |
|
Researchers
| سمر حسن قحطان | Qahtan, Samar Hassan | Researcher | Master | |
|
Back To Researches Page
|