Document Details
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Document Type |
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Thesis |
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Document Title |
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Prevalence and Molecular Characterization of Carbapenem-Resistant Gram-Negative Bacteria in Patients at King Faisal Hospital, Taif, Saudi Arabia انتشار والخصائص الجزيئية للبكتيريا سالبه الجرام المقاومة للمضاد الحيوي الكاربابينيم في مرضى مستشفى الملك فيصل في محافظة الطائف بالمملكة العربية السعودية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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This study aims to identify carbapenem-resistant Gram-negative bacterial infection (CR-GNB) prevalence in King Faisal Medical Complex Hospital (KFMC), at Taif, Saudi Arabia, and to determine the distribution of biotypes, antibiotypes, site of infection, hospital wards, multiple associated demographics, clinical characteristics, comorbidity risk factors, carbapenemase genes and ability to biofilm formation. Clinical samples were obtained from patients admitted to KFMC, over a period of 6 months (November 2021 to April 2022) and were screened for carbapenem resistant (CR) by Disk Diffusion method and Phoenix System. Patients' demographic and comorbidity data were collected. Further, the detection of carbapenemase producer (CP) genes was performed using the X΄pert Carba-R molecular method. The ability to biofilm formation was measured by crystal violet staining method. Overall, 763 clinical infections by Gram-negative isolates were identified, 236 (32%) of the clinical isolates were determined as CR-GNB, the most isolates came from blood, (n=71/236, 30.1%), then from sputum (n=63/236, 26.7%). The highest incidences of CR infections were recorded in intensive care units (ICU) (25.48%) and in Klebsiella spp. (65.7%), Acinetobacter baumannii (16.1%), and Pseudomonas spp. (12.7%). The tested CR isolates were classified into 29 antibiotypes patterns. The CR-GNB infection increased at the high age, male gender, and long hospitalization of the patient and there were significant association between CR-GNB infection and cardiovascular, pulmonary, neurologic, renal, bed sores, hepatic, and malignancy disease. The most prevalent clinical characteristics were urinary catheter insertion (72.5%), invasive procedure (70.1%), artificial ventilation (65.3%), ICU administration (61.7%), and dialysis (14.4%). CR-GNB infection increased in COVID-19 patients (40.7%) and death rate among CR patients was 40.7%. Many of CR isolates didn’t have any carbapenemase genes (41.3%) and no isolates were positive for blaKPC, blaVIM and blaIMP. There were three distinct groups of CP isolates circulating in KFMC. The first was positive for blaNDM 42 (25.1 %). The second was positive for blaOXA (30, 18.6%). The third was positive for both blaOXA and blaNDM (26, 15.6%). Most CR-GNB isolates were strong biofilm former (77.8%). In conclusion, there is a great need to find out the source of infection and prevalence of CR-GNB in infected and carrier patients around KSA to assess differences CP genes sequencing between the regions to determine the epidemiological status.
Keywords: Carbapenemases, Risk factors, CRE, Carbapenem resistance, biofilm. |
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Supervisor |
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Prof. Dr. Magda Mohammed Ali |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Dr. Reda Amashah |
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Added Date |
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Wednesday, November 1, 2023 |
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Researchers
| روان سامي أبو عاصي | Abou assy, Rawan Sami | Researcher | Doctorate | |
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