Document Details
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Document Type |
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Thesis |
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Document Title |
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Synthesis, Characterization, Biological Activity and Molecular Modeling Studies of Some Transition Metals Complexes of Azo Dye Ligand تحضير، توصيف ودراسة النشاط البيولوجي والشكل الجزيئي لمعقدات بعض العناصر الانتقالية مع ليجاند صبغة الآزو |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Presently, the rising number of cancer cases is a worrying trend, making it the leading cause of premature death in most developed and developing countries. Infectious diseases can also cause severe and even life-threatening. Azo dye compounds with transition metals have been played an important role in the biological systems which, caught the interest of researchers. In the present work, we aimed to synthesize novel biologically active azo dye compounds which have anti-cancer and/or antimicrobial characteristics. During conducting this study, three novel azo dye ligands containing different naphthalene derivatives were synthesized via a diazo-coupling reaction. These method offers several advantages, including: (1) quick to respond, (2) wide application range, (3) possessing high yield, and (4) mild reaction conditions. The structures of the synthesized compounds were characterized using several physical techniques including: FTIR, 1H NMR, electronic spectra, and thermal analysis. The morphology of compounds obtained from X-ray Diffractometry (XRD) and Scanning Electron Microscopy (SEM) systems were presented. Moreover, the density functional theory (DFT) technique has been used to investigate the structure, stability, and electronic properties. The biocompatibility was investigated by MTT assay, DNA fragmentation, and agar diffusion assay. The data showed that the proposed complexes have an octahedral geometry around the central metal ion. The non‐electrolytic nature of the complexes was confirmed by molar conductance measurement. Furthermore, X-ray powder diffraction indicates the average crystallite size using the Scherer method and the crystal systems of investigated metal complexes. The bond strength and molecular stability between the interaction of metal ions and the ligand were investigated by density functional theory (DFT). The results of bond lengths and the bond angles of the investigated compounds in good agreement with octahedral geometry for all complexes. The results indicated that some compounds exhibited antimicrobial activity, some had lower activity, and others had none. The results showed that (HL3) has the highest effective with (IC50 = 22.1 µM) than the other two prepared ligands but their metal complexes are not. However, the (HL2) the only ligand which increases its activity during the complexation. DNA fragments for all synthesized ligands and their complexes were estimated, and showed extensive DNA damage of HepG2 cancer cell at high concentration compared with the control, P ≤ 0.001. |
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Supervisor |
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Prof. Mutlaq Shediad Aljahdali |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Prof. Abdulmohsen Ali Alshehri |
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Added Date |
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Saturday, November 4, 2023 |
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Researchers
| البندري فؤاد الكردي | Alkurdi, Albandari Fouad | Researcher | Doctorate | |
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