Document Details

Document Type : Thesis 
Document Title :
Effect of Environmental Pollution by Crude Oil on Seabass, Dicentrarchus labrax: Histological Studies
تأثير التلوث البيئي بزيت البترول الخام على أسماك القاروص: دراسة نسيجية
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : The objective of this study was to determine the effects of the dispersant crude oil on the exposure and toxicity to fish. The study aims to evaluate the effects induced due to marine pollution by the crude oil on the histological pattern and the biochemical parameters of one of the most common fish in the red sea, the sea bass, Dicentrarchus labrax. Mortality rates were higher in the group of fishes exposed to the highest concentration (0.8ml/l crude petroleum), reaching 100 percent at the end of the experimental duration (10 days). These results indicate that fish exhibited a progressive fall in the haemoglobin content as 10.42, 3.19 and 3.12 in groups of fishes subjected to control, 0.4 and 0.2 ml/l crude petroleum reached to non detectable value at 0.8 ml/l concentration. There was a progressive significant increase in mortality rate with the laps of time compared to the median and lowest concentrations and control groups (P< 0.001). In the highest dose (0.8 ml/l) group, serum AST and ALT were significantly increased as compared to lowest dose (0.2 ml/l) and control group (p<0.001). The elevated activities of serum AST and ALT were significantly increased in all treated animal groups with the laps of time. Such increase was directly proportional to the concentration of the pollutant. There were highly significant changes (P< 0.001) in the levels of glutathione (GSH) in the blood when compared to control values. Gills from fish exposed to crude oil in all of the contaminated groups (2%, 4% and 8% crude oil) showed a higher prevalence of aneurysms and especially hyperplasia and fusion of the secondary lamellae than fish of the control group. Edema with lifting of the secondary lamellar epithelium was often observed in association with epithelial hyperplasia. Liver of 8% crude oil, showing large vacuoles, necrosis, and proliferation of hepatic cells compared with control group. The hepatocytes of crude oil exposed fish had a polygonal shape and contained perinuclear rough endoplasmic reticulum associated with branched tubular smooth endoplasmic reticulum at its periphery. Light Microscope investigations of the kidney sections of control and crude oil fishes showing certain degenerated urineferous tubules and dilatation of Bowman's capsule compared with control group which show Normal renal tissues showing normal urineferous tubules and glomeruli. In response to crude oil exposure, ultramorphologic analysis revealed that a prominent thickening of the basal laminae, a shortening of pedicels, and their retraction from the basal lamina became evident in the renal corpuscles compared with control group. 
Supervisor : Prof. Osama Abdullah Abu-Zinadah 
Thesis Type : Doctorate Thesis 
Publishing Year : 1433 AH
2012 AD 
Co-Supervisor : Prof. Hussein Khamis Hussein Ali . Prof. Tarek Rashed Rahmy 
Added Date : Sunday, September 2, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبد القادر محمد شيخ عمرShaikh Omar, Abdul-kader MOHAMMADResearcherDoctorate 

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